Detailed information of TA system TA128838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 19373..19871
• Replicon: plasmid p35K_like
• Accession: NZ_CP041625
• Organism: Escherichia coli O157:H7 strain ATCC 43888

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A764VNK7
• Locus tag: FNZ21_RS27680
• Protein ID: WP_000868275.1
• Coordinates: 19373..19639 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: A0A3H7VMJ5
• Locus tag: FNZ21_RS27685
• Protein ID: WP_001450803.1
• Coordinates: 19629..19871 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ21_RS27630	14389..14622	+	234	WP_001056371.1	EexN family lipoprotein	-
FNZ21_RS27950	14663..14950	-	288	WP_000332251.1	helix-turn-helix transcriptional regulator	-
FNZ21_RS27640	15149..15487	+	339	WP_000612441.1	helix-turn-helix domain-containing protein	-
FNZ21_RS27645	15729..16733	-	1005	WP_001208090.1	replication initiation protein	-
FNZ21_RS27665	18414..18737	+	324	WP_000432877.1	hypothetical protein	-
FNZ21_RS27670	18778..19002	+	225	WP_000866040.1	hypothetical protein	-
FNZ21_RS27675	19098..19322	+	225	WP_000749827.1	hypothetical protein	-
FNZ21_RS27680	19373..19639	+	267	WP_000868275.1	hypothetical protein	Toxin
FNZ21_RS27685	19629..19871	+	243	WP_001450803.1	hypothetical protein	Antitoxin
FNZ21_RS27695	20311..20640	+	330	WP_000449469.1	hypothetical protein	-
FNZ21_RS27700	20698..20976	+	279	WP_000545934.1	hypothetical protein	-
FNZ21_RS27705	21406..21588	+	183	WP_000833471.1	type II toxin-antitoxin system HicA family toxin	-
FNZ21_RS27710	21613..22050	+	438	WP_000466317.1	type II toxin-antitoxin system HicB family antitoxin	-
FNZ21_RS27715	22079..22783	-	705	WP_001279868.1	hypothetical protein	-
FNZ21_RS27720	22780..23541	-	762	WP_001097937.1	conjugal transfer protein TraL	-
FNZ21_RS27725	23551..23958	-	408	WP_001190619.1	hypothetical protein	-
FNZ21_RS27730	24304..24639	+	336	WP_000963830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32726	32726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10199.63 Da
• Isoelectric Point: 9.5657

>T128838 WP_000868275.1 NZ_CP041625:19373-19639 [Escherichia coli O157:H7]
MKVRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Nucleotide

• Length: 267 bp

>T128838 NZ_CP041625:19373-19639 [Escherichia coli O157:H7]
ATGAAGGTACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTGATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9288.69 Da
• Isoelectric Point: 9.7120

>AT128838 WP_001450803.1 NZ_CP041625:19629-19871 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Nucleotide

• Length: 243 bp

>AT128838 NZ_CP041625:19629-19871 [Escherichia coli O157:H7]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A764VNK7

Antitoxin

• AlphaFold DB: A0A3H7VMJ5