Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 74270..74829 | Replicon | plasmid pO157_like |
| Accession | NZ_CP041624 | ||
| Organism | Escherichia coli O157:H7 strain ATCC 43888 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | FNZ21_RS27450 | Protein ID | WP_000421248.1 |
| Coordinates | 74554..74829 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | FNZ21_RS27445 | Protein ID | WP_001178089.1 |
| Coordinates | 74270..74554 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FNZ21_RS27390 | 69462..70022 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| FNZ21_RS27395 | 70155..70367 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| FNZ21_RS27400 | 70612..71073 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| FNZ21_RS27405 | 71119..71328 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| FNZ21_RS27410 | 71366..71704 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| FNZ21_RS27415 | 71944..72198 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| FNZ21_RS27425 | 72434..72508 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| FNZ21_RS27430 | 72501..73358 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| FNZ21_RS27445 | 74270..74554 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| FNZ21_RS27450 | 74554..74829 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| FNZ21_RS27455 | 74924..75130 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| FNZ21_RS27460 | 75230..75445 | - | 216 | Protein_83 | transposase | - |
| FNZ21_RS27465 | 75489..76055 | - | 567 | Protein_84 | IS3-like element IS600 family transposase | - |
| FNZ21_RS27470 | 76055..76471 | - | 417 | Protein_85 | IS91 family transposase | - |
| FNZ21_RS27475 | 76471..76656 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| FNZ21_RS27480 | 76833..79043 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| FNZ21_RS27485 | 79087..79476 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | exeF / exeG / hlyC / hlyA / hlyA / hlyB / hlyD / toxB / espP | 1..95081 | 95081 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T128837 WP_000421248.1 NZ_CP041624:74554-74829 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T128837 NZ_CP041624:74554-74829 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT128837 WP_001178089.1 NZ_CP041624:74270-74554 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT128837 NZ_CP041624:74270-74554 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |