Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4120267..4120850 Replicon chromosome
Accession NZ_CP041620
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag FNL95_RS21100 Protein ID WP_000254749.1
Coordinates 4120515..4120850 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FNL95_RS21095 Protein ID WP_000581937.1
Coordinates 4120267..4120515 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS21085 4116606..4117907 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FNL95_RS21090 4117955..4120189 + 2235 WP_000226797.1 GTP diphosphokinase -
FNL95_RS21095 4120267..4120515 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FNL95_RS21100 4120515..4120850 + 336 WP_000254749.1 endoribonuclease MazF Toxin
FNL95_RS21105 4120922..4121713 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FNL95_RS21110 4121941..4123578 + 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
FNL95_RS21115 4123666..4124964 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T128798 WP_000254749.1 NZ_CP041620:4120515-4120850 [Shigella flexneri]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T128798 NZ_CP041620:4120515-4120850 [Shigella flexneri]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT128798 WP_000581937.1 NZ_CP041620:4120267-4120515 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT128798 NZ_CP041620:4120267-4120515 [Shigella flexneri]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References