Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3964272..3964926 Replicon chromosome
Accession NZ_CP041620
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FNL95_RS20460 Protein ID WP_000244781.1
Coordinates 3964519..3964926 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FNL95_RS20455 Protein ID WP_000354046.1
Coordinates 3964272..3964538 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS20435 3960360..3961793 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
FNL95_RS20440 3961838..3962149 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
FNL95_RS20445 3962313..3962972 + 660 WP_000250274.1 hemolysin III family protein -
FNL95_RS20450 3963049..3964029 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
FNL95_RS20455 3964272..3964538 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FNL95_RS20460 3964519..3964926 + 408 WP_000244781.1 protein YgfX Toxin
FNL95_RS20465 3964966..3965487 - 522 WP_001055874.1 flavodoxin FldB -
FNL95_RS20470 3965599..3966495 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FNL95_RS20475 3966520..3967230 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FNL95_RS20480 3967236..3968969 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T128797 WP_000244781.1 NZ_CP041620:3964519-3964926 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T128797 NZ_CP041620:3964519-3964926 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT128797 WP_000354046.1 NZ_CP041620:3964272-3964538 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT128797 NZ_CP041620:3964272-3964538 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References