Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2464318..2464815 Replicon chromosome
Accession NZ_CP041620
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FNL95_RS13225 Protein ID WP_001173343.1
Coordinates 2464318..2464491 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag FNL95_RS13230 Protein ID WP_001312331.1
Coordinates 2464519..2464815 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS13215 2460988..2462445 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
FNL95_RS13220 2462682..2464199 + 1518 WP_000003806.1 lysine--tRNA ligase -
FNL95_RS13225 2464318..2464491 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FNL95_RS13230 2464519..2464815 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FNL95_RS13235 2465044..2465316 - 273 WP_000405647.1 N-acetyltransferase -
FNL95_RS13240 2465328..2465558 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
FNL95_RS13245 2465739..2467370 + 1632 WP_001216482.1 sensor histidine kinase -
FNL95_RS13250 2467367..2468086 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T128792 WP_001173343.1 NZ_CP041620:c2464491-2464318 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T128792 NZ_CP041620:c2464491-2464318 [Shigella flexneri]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT128792 WP_001312331.1 NZ_CP041620:c2464815-2464519 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT128792 NZ_CP041620:c2464815-2464519 [Shigella flexneri]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References