Detailed information of TA system TA128791

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2452523..2453355
• Replicon: chromosome
• Accession: NZ_CP041620
• Organism: Shigella flexneri strain C32

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: FNL95_RS13170
• Protein ID: WP_000854753.1
• Coordinates: 2452981..2453355 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: FNL95_RS13165
• Protein ID: WP_001540478.1
• Coordinates: 2452523..2452891 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNL95_RS13130	2448356..2449036	+	681	WP_001282919.1	WYL domain-containing protein	-
FNL95_RS13135	2449184..2449861	+	678	WP_001097301.1	hypothetical protein	-
FNL95_RS13140	2449867..2450100	+	234	WP_001278283.1	DUF905 family protein	-
FNL95_RS13145	2450190..2451008	+	819	WP_001773857.1	DUF945 domain-containing protein	-
FNL95_RS13150	2451099..2451584	+	486	WP_000214398.1	antirestriction protein	-
FNL95_RS13155	2451600..2452076	+	477	WP_001186786.1	RadC family protein	-
FNL95_RS13160	2452139..2452360	+	222	WP_000692311.1	DUF987 domain-containing protein	-
FNL95_RS13165	2452523..2452891	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNL95_RS13170	2452981..2453355	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
FNL95_RS13175	2453352..2453840	+	489	WP_000777545.1	hypothetical protein	-
FNL95_RS13180	2453852..2454049	+	198	WP_000839281.1	DUF957 domain-containing protein	-
FNL95_RS13185	2454146..2454715	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
FNL95_RS13195	2455464..2457002	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2408744..2457002	48258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T128791 WP_000854753.1 NZ_CP041620:2452981-2453355 [Shigella flexneri]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T128791 NZ_CP041620:2452981-2453355 [Shigella flexneri]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT128791 WP_001540478.1 NZ_CP041620:2452523-2452891 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128791 NZ_CP041620:2452523-2452891 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68