128783

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2040138..2040688 Replicon chromosome
Accession NZ_CP041620
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag FNL95_RS11025 Protein ID WP_001160966.1
Coordinates 2040138..2040452 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag FNL95_RS11030 Protein ID WP_000125568.1
Coordinates 2040455..2040688 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS11000 2035813..2037099 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
FNL95_RS11005 2037099..2038088 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
FNL95_RS11010 2038085..2038906 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
FNL95_RS11015 2038909..2039286 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
FNL95_RS11020 2039293..2040141 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
FNL95_RS11025 2040138..2040452 - 315 WP_001160966.1 CcdB family protein Toxin
FNL95_RS11030 2040455..2040688 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FNL95_RS11035 2040774..2041253 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
FNL95_RS11040 2041445..2043307 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
FNL95_RS11045 2043300..2043830 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
FNL95_RS11050 2043938..2045269 - 1332 WP_001183207.1 MFS transporter -
FNL95_RS11055 2045328..2045615 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T128783 WP_001160966.1 NZ_CP041620:c2040452-2040138 [Shigella flexneri]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T128783 NZ_CP041620:c2040452-2040138 [Shigella flexneri]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT128783 WP_000125568.1 NZ_CP041620:c2040688-2040455 [Shigella flexneri]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT128783 NZ_CP041620:c2040688-2040455 [Shigella flexneri]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References