128770

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 83927..84452 Replicon plasmid pC32_1
Accession NZ_CP041619
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FNL95_RS00610 Protein ID WP_001159868.1
Coordinates 83927..84232 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FNL95_RS00615 Protein ID WP_000813634.1
Coordinates 84234..84452 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS00595 79837..81003 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FNL95_RS00600 81591..82346 - 756 WP_000852146.1 replication initiation protein RepE -
FNL95_RS00605 83120..83926 - 807 WP_000016982.1 site-specific integrase -
FNL95_RS00610 83927..84232 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FNL95_RS00615 84234..84452 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FNL95_RS00620 85019..85531 + 513 WP_000151784.1 hypothetical protein -
FNL95_RS00625 85565..86698 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
FNL95_RS00630 86865..87638 - 774 WP_000905949.1 hypothetical protein -
FNL95_RS00635 87651..88151 - 501 WP_001773886.1 hypothetical protein -
FNL95_RS00640 88416..88646 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FNL95_RS00645 88643..89059 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id senB 1..90682 90682


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T128770 WP_001159868.1 NZ_CP041619:c84232-83927 [Shigella flexneri]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T128770 NZ_CP041619:c84232-83927 [Shigella flexneri]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT128770 WP_000813634.1 NZ_CP041619:c84452-84234 [Shigella flexneri]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT128770 NZ_CP041619:c84452-84234 [Shigella flexneri]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References