Detailed information of TA system TA128757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 92820..93408
• Replicon: plasmid pJ9-3
• Accession: NZ_CP041590
• Organism: Acinetobacter baumannii strain J9

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: FK728_RS18645
• Protein ID: WP_000438825.1
• Coordinates: 92820..93107 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: FK728_RS18650
• Protein ID: WP_004728120.1
• Coordinates: 93094..93408 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK728_RS18620	88375..89259	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
FK728_RS18625	89315..90790	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FK728_RS18630	91283..91555	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FK728_RS18635	91548..91868	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FK728_RS18640	92021..92551	+	531	WP_000172759.1	hypothetical protein	-
FK728_RS18645	92820..93107	+	288	WP_000438825.1	BrnT family toxin	Toxin
FK728_RS18650	93094..93408	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
FK728_RS18655	93475..93771	-	297	WP_111916319.1	MerR family DNA-binding protein	-
FK728_RS18660	93797..94180	+	384	WP_001055585.1	IS66 family insertion sequence hypothetical protein	-
FK728_RS18665	94177..94512	+	336	WP_000618092.1	IS66 family insertion sequence element accessory protein TnpB	-
FK728_RS18670	94587..96170	+	1584	WP_111916287.1	IS66-like element ISAba25 family transposase	-
FK728_RS18675	96381..97067	-	687	WP_111916318.1	hypothetical protein	-
FK728_RS18680	97134..97733	-	600	WP_109394146.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / blaADC-25 / mph(E) / msr(E)	-	1..145071	145071
	-	inside	IScluster/Tn	floR / blaADC-25 / mph(E) / msr(E)	-	79391..98850	19459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T128757 WP_000438825.1 NZ_CP041590:92820-93107 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T128757 NZ_CP041590:92820-93107 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT128757 WP_004728120.1 NZ_CP041590:93094-93408 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT128757 NZ_CP041590:93094-93408 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9