Detailed information of TA system TA128755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2905..3493
• Replicon: plasmid pJ9-2
• Accession: NZ_CP041589
• Organism: Acinetobacter baumannii strain J9

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: FK728_RS18075
• Protein ID: WP_000438825.1
• Coordinates: 2905..3192 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: FK728_RS18080
• Protein ID: WP_061855757.1
• Coordinates: 3179..3493 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK728_RS18060	144..1094	+	951	WP_001208778.1	replication initiation protein RepM	-
FK728_RS18065	1087..1662	+	576	WP_001096619.1	helix-turn-helix domain-containing protein	-
FK728_RS19015	1682..1825	+	144	WP_001125246.1	hypothetical protein	-
FK728_RS18070	2014..2532	-	519	WP_000447193.1	sel1 repeat family protein	-
FK728_RS18075	2905..3192	+	288	WP_000438825.1	BrnT family toxin	Toxin
FK728_RS18080	3179..3493	+	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
FK728_RS18085	3621..6032	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
FK728_RS18090	6337..6798	+	462	WP_000761346.1	DIP1984 family protein	-
FK728_RS18095	7234..8259	-	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10967	10967
	-	flank	IS/Tn	-	-	7234..8259	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T128755 WP_000438825.1 NZ_CP041589:2905-3192 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T128755 NZ_CP041589:2905-3192 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT128755 WP_061855757.1 NZ_CP041589:3179-3493 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT128755 NZ_CP041589:3179-3493 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A858UV00