128749

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5671031..5671578 Replicon chromosome
Accession NZ_CP041515
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag FNA74_RS28695 Protein ID WP_014227299.1
Coordinates 5671270..5671578 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag FNA74_RS28690 Protein ID WP_014227298.1
Coordinates 5671031..5671267 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS28675 5667238..5668404 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
FNA74_RS28680 5668565..5669467 + 903 WP_064375676.1 N-acetylmuramic acid 6-phosphate etherase -
FNA74_RS28685 5669492..5670925 + 1434 WP_048262922.1 PTS N-acetylmuramic acid transporter subunit IIBC -
FNA74_RS28690 5671031..5671267 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FNA74_RS28695 5671270..5671578 + 309 WP_014227299.1 CcdB family protein Toxin
FNA74_RS28700 5671729..5672630 + 902 Protein_5267 EamA family transporter -
FNA74_RS28705 5672634..5673599 - 966 WP_064375675.1 Rpn family recombination-promoting nuclease/putative transposase -
FNA74_RS28710 5673809..5674102 + 294 WP_014227302.1 hypothetical protein -
FNA74_RS28715 5674126..5675067 - 942 WP_014227303.1 LacI family transcriptional regulator -
FNA74_RS28720 5675362..5675673 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T128749 WP_014227299.1 NZ_CP041515:5671270-5671578 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T128749 NZ_CP041515:5671270-5671578 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT128749 WP_014227298.1 NZ_CP041515:5671031-5671267 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT128749 NZ_CP041515:5671031-5671267 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTTAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References