Detailed information of TA system TA128746

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4799320..4800124
• Replicon: chromosome
• Accession: NZ_CP041515
• Organism: Klebsiella michiganensis strain KNU07

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: FNA74_RS24465
• Protein ID: WP_047683725.1
• Coordinates: 4799320..4799709 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FNA74_RS24470
• Protein ID: WP_047683727.1
• Coordinates: 4799765..4800124 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA74_RS24435	4794423..4795070	-	648	WP_064358907.1	LysE family translocator	-
FNA74_RS24440	4795175..4796026	+	852	WP_064358883.1	aldo/keto reductase	-
FNA74_RS24445	4796053..4797042	+	990	WP_049083229.1	aldo/keto reductase	-
FNA74_RS24450	4797077..4797970	-	894	WP_049083228.1	LysR family transcriptional regulator	-
FNA74_RS24460	4798399..4799238	-	840	WP_047683720.1	DUF4942 domain-containing protein	-
FNA74_RS24465	4799320..4799709	-	390	WP_047683725.1	TA system toxin CbtA family protein	Toxin
FNA74_RS24470	4799765..4800124	-	360	WP_047683727.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNA74_RS24475	4800149..4800370	-	222	WP_014837279.1	DUF987 domain-containing protein	-
FNA74_RS24480	4800384..4800863	-	480	WP_014837278.1	DNA repair protein RadC	-
FNA74_RS24485	4800875..4801318	-	444	WP_014837277.1	antirestriction protein	-
FNA74_RS24490	4801398..4801646	-	249	WP_044347584.1	DUF905 domain-containing protein	-
FNA74_RS24495	4801652..4802476	-	825	WP_040217125.1	DUF945 domain-containing protein	-
FNA74_RS24500	4803019..4803402	+	384	WP_143444486.1	inovirus-type Gp2 protein	-
FNA74_RS24505	4803447..4804414	-	968	Protein_4451	IS5 family transposase	-
FNA74_RS24510	4804434..4804715	+	282	WP_143444545.1	inovirus-type Gp2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-110)

Antitoxin

• Antitoxin CbeA (16-114)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14426.58 Da
• Isoelectric Point: 9.1379

>T128746 WP_047683725.1 NZ_CP041515:c4799709-4799320 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNCGTYKDVTAITRGLHRQVSTPGKR

Nucleotide

• Length: 390 bp

>T128746 NZ_CP041515:c4799709-4799320 [Klebsiella michiganensis]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAATTGTGG
CACGTATAAAGATGTGACGGCCATTACCAGAGGACTGCATAGACAGGTCAGCACCCCCGGTAAACGCTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13474.28 Da
• Isoelectric Point: 6.7394

>AT128746 WP_047683727.1 NZ_CP041515:c4800124-4799765 [Klebsiella michiganensis]
MSKKTTIETHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Nucleotide

• Length: 360 bp

>AT128746 NZ_CP041515:c4800124-4799765 [Klebsiella michiganensis]
TTGTCGAAGAAAACCACGATAGAAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA