Detailed information of TA system TA128745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3696145..3696735
• Replicon: chromosome
• Accession: NZ_CP041515
• Organism: Klebsiella michiganensis strain KNU07

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: FNA74_RS19190
• Protein ID: WP_064375864.1
• Coordinates: 3696145..3696477 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FNA74_RS19195
• Protein ID: WP_064375863.1
• Coordinates: 3696478..3696735 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA74_RS19160	3691559..3691816	+	258	WP_014229908.1	histidine kinase	-
FNA74_RS19165	3691949..3693403	+	1455	WP_014229909.1	AMP nucleosidase	-
FNA74_RS19180	3694365..3695822	-	1458	WP_025106072.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
FNA74_RS19190	3696145..3696477	-	333	WP_064375864.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FNA74_RS19195	3696478..3696735	-	258	WP_064375863.1	hypothetical protein	Antitoxin
FNA74_RS19205	3697087..3697280	-	194	Protein_3439	helix-turn-helix domain-containing protein	-
FNA74_RS19210	3697277..3697738	-	462	WP_064375862.1	hypothetical protein	-
FNA74_RS19220	3698214..3699419	+	1206	WP_064375861.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11853.73 Da
• Isoelectric Point: 10.5834

>T128745 WP_064375864.1 NZ_CP041515:c3696477-3696145 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGANFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T128745 NZ_CP041515:c3696477-3696145 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGCAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGACTGCCCGGAACGGCAAGCGGCTGGAACGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9463.89 Da
• Isoelectric Point: 4.3219

>AT128745 WP_064375863.1 NZ_CP041515:c3696735-3696478 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPYAEMSEEDREWIDAPAV
GKEIQ

Nucleotide

• Length: 258 bp

>AT128745 NZ_CP041515:c3696735-3696478 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGTACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCAGTAG