Detailed information of TA system TA128734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 416853..417429
• Replicon: chromosome
• Accession: NZ_CP041515
• Organism: Klebsiella michiganensis strain KNU07

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FNA74_RS03245
• Protein ID: WP_064375702.1
• Coordinates: 416853..417140 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: FNA74_RS03250
• Protein ID: WP_014227757.1
• Coordinates: 417127..417429 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA74_RS03215	412198..412716	-	519	WP_004098240.1	hypothetical protein	-
FNA74_RS03220	412713..413513	-	801	WP_064375698.1	helix-turn-helix domain-containing protein	-
FNA74_RS03225	413659..414242	-	584	Protein_370	TetR/AcrR family transcriptional regulator	-
FNA74_RS03230	414390..415172	-	783	WP_064375699.1	NAD(P)H-dependent oxidoreductase	-
FNA74_RS03235	415260..416168	+	909	WP_064375700.1	LysR family transcriptional regulator	-
FNA74_RS03240	416162..416605	+	444	WP_064375701.1	FosA family fosfomycin resistance glutathione transferase	-
FNA74_RS03245	416853..417140	+	288	WP_064375702.1	BrnT family toxin	Toxin
FNA74_RS03250	417127..417429	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
FNA74_RS03255	417550..418893	-	1344	WP_049101852.1	APC family permease	-
FNA74_RS03260	418890..419801	-	912	WP_049080536.1	acetamidase/formamidase family protein	-
FNA74_RS03265	420257..420592	-	336	WP_064375703.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.69 Da
• Isoelectric Point: 7.4687

>T128734 WP_064375702.1 NZ_CP041515:416853-417140 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T128734 NZ_CP041515:416853-417140 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT128734 WP_014227757.1 NZ_CP041515:417127-417429 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT128734 NZ_CP041515:417127-417429 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591