Detailed information of TA system TA128733

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 361334..362037
• Replicon: chromosome
• Accession: NZ_CP041515
• Organism: Klebsiella michiganensis strain KNU07

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: FNA74_RS02990
• Protein ID: WP_143444272.1
• Coordinates: 361696..362037 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: FNA74_RS02985
• Protein ID: WP_143444270.1
• Coordinates: 361334..361675 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA74_RS02950	356487..357362	+	876	WP_023303014.1	GTPase family protein	-
FNA74_RS02955	357606..358322	+	717	WP_064736452.1	WYL domain-containing protein	-
FNA74_RS02960	358358..358810	+	453	WP_143444260.1	hypothetical protein	-
FNA74_RS02965	358882..359355	+	474	WP_143444262.1	hypothetical protein	-
FNA74_RS02970	359476..360297	+	822	WP_143444264.1	DUF945 domain-containing protein	-
FNA74_RS02975	360328..360768	+	441	WP_143444266.1	antirestriction protein	-
FNA74_RS02980	360781..361323	+	543	WP_143444268.1	DNA repair protein RadC	-
FNA74_RS02985	361334..361675	+	342	WP_143444270.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNA74_RS02990	361696..362037	+	342	WP_143444272.1	TA system toxin CbtA family protein	Toxin
FNA74_RS02995	362287..363294	+	1008	WP_143444274.1	restriction endonuclease	-
FNA74_RS03000	363523..364266	-	744	WP_064375729.1	hypothetical protein	-
FNA74_RS03005	364432..364866	-	435	WP_064375730.1	VOC family protein	-
FNA74_RS03010	365012..365335	-	324	WP_064375731.1	endoribonuclease SymE	-
FNA74_RS03015	365625..366329	-	705	WP_077265718.1	DNA/RNA non-specific endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	356487..365335	8848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12620.58 Da
• Isoelectric Point: 8.9922

>T128733 WP_143444272.1 NZ_CP041515:361696-362037 [Klebsiella michiganensis]
MKTLPATTPQAATLCLSPVAVWKMLIARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLGAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T128733 NZ_CP041515:361696-362037 [Klebsiella michiganensis]
ATGAAAACTTTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGAAAATGCTGATTGC
ACGCCTGCTGGAACAGCATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAGACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGGGGGCAGTCGATATTCTGAGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12851.36 Da
• Isoelectric Point: 5.8775

>AT128733 WP_143444270.1 NZ_CP041515:361334-361675 [Klebsiella michiganensis]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLVDRASITGEFSNAESLKLDEVFPHFISQMESMWTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT128733 NZ_CP041515:361334-361675 [Klebsiella michiganensis]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGTTGACCGGGCCAGCATTACCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTGGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA