Detailed information of TA system TA128732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 149852..150378
• Replicon: plasmid unnamed
• Accession: NZ_CP041514
• Organism: Klebsiella michiganensis strain KNU07

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FNA74_RS00910
• Protein ID: WP_000323025.1
• Coordinates: 149852..150139 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: FNA74_RS00915
• Protein ID: WP_004196370.1
• Coordinates: 150139..150378 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA74_RS00865	145167..145391	-	225	WP_014343499.1	hypothetical protein	-
FNA74_RS00870	145402..145614	-	213	WP_019706020.1	hypothetical protein	-
FNA74_RS00875	145675..146031	-	357	WP_019706019.1	hypothetical protein	-
FNA74_RS00885	146872..147189	-	318	WP_015065519.1	hypothetical protein	-
FNA74_RS00890	147204..147554	-	351	WP_032425551.1	hypothetical protein	-
FNA74_RS00895	147551..147823	-	273	WP_032425552.1	hypothetical protein	-
FNA74_RS00900	148521..148679	-	159	WP_014343509.1	type I toxin-antitoxin system Hok family toxin	-
FNA74_RS00905	148751..149785	+	1035	Protein_174	IS481-like element ISKpn28 family transposase	-
FNA74_RS00910	149852..150139	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FNA74_RS00915	150139..150378	-	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FNA74_RS00920	150629..150994	+	366	WP_009651956.1	hypothetical protein	-
FNA74_RS00925	151038..151775	+	738	WP_008460272.1	HupE/UreJ family protein	-
FNA74_RS00930	151789..152478	+	690	WP_004196322.1	hypothetical protein	-
FNA74_RS00935	152509..153885	-	1377	Protein_180	chromate efflux transporter	-
FNA74_RS00940	153842..154819	-	978	WP_004196334.1	chromate resistance protein	-
FNA74_RS00945	154849..155041	+	193	Protein_182	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..203764	203764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T128732 WP_000323025.1 NZ_CP041514:c150139-149852 [Klebsiella michiganensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T128732 NZ_CP041514:c150139-149852 [Klebsiella michiganensis]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT128732 WP_004196370.1 NZ_CP041514:c150378-150139 [Klebsiella michiganensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT128732 NZ_CP041514:c150378-150139 [Klebsiella michiganensis]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4