128731

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 60196..60932 Replicon plasmid unnamed
Accession NZ_CP041514
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag FNA74_RS00370 Protein ID WP_003026803.1
Coordinates 60450..60932 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FNA74_RS00365 Protein ID WP_003026799.1
Coordinates 60196..60462 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS00315 56025..56240 - 216 Protein_60 AAA family ATPase -
FNA74_RS00320 56258..56620 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
FNA74_RS00325 56670..57020 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
FNA74_RS00330 57378..57647 + 270 WP_004152102.1 hypothetical protein -
FNA74_RS00335 57635..58210 + 576 WP_004152103.1 hypothetical protein -
FNA74_RS00340 58241..58735 + 495 WP_004152104.1 DNA-binding protein -
FNA74_RS00345 58779..59147 + 369 WP_004152105.1 hypothetical protein -
FNA74_RS00350 59181..59384 + 204 WP_004152106.1 hemolysin expression modulator Hha -
FNA74_RS00355 59433..59690 + 258 WP_004152107.1 hypothetical protein -
FNA74_RS00360 59766..60020 + 255 WP_004152108.1 hypothetical protein -
FNA74_RS00365 60196..60462 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FNA74_RS00370 60450..60932 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
FNA74_RS00375 61140..62486 + 1347 WP_077253535.1 ISNCY family transposase -
FNA74_RS00380 62535..62930 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
FNA74_RS00385 63128..64474 + 1347 WP_077255298.1 ISNCY family transposase -
FNA74_RS00390 64533..65207 + 675 Protein_75 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..203764 203764
- flank IS/Tn - - 54957..55961 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T128731 WP_003026803.1 NZ_CP041514:60450-60932 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T128731 NZ_CP041514:60450-60932 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT128731 WP_003026799.1 NZ_CP041514:60196-60462 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT128731 NZ_CP041514:60196-60462 [Klebsiella michiganensis]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References