Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14173..14816 Replicon plasmid unnamed
Accession NZ_CP041514
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FNA74_RS00110 Protein ID WP_001044770.1
Coordinates 14400..14816 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FNA74_RS00105 Protein ID WP_001261282.1
Coordinates 14173..14403 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS00075 10293..10928 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
FNA74_RS00080 11036..11380 - 345 WP_009309911.1 hypothetical protein -
FNA74_RS00085 11702..12638 + 937 Protein_14 CAAX protease -
FNA74_RS00090 12829..13269 - 441 WP_047057603.1 hypothetical protein -
FNA74_RS00095 13288..13881 - 594 WP_009309909.1 hypothetical protein -
FNA74_RS00100 13980..14216 - 237 Protein_17 hypothetical protein -
FNA74_RS00105 14173..14403 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FNA74_RS00110 14400..14816 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FNA74_RS00115 14890..16452 + 1563 WP_009309907.1 AAA family ATPase -
FNA74_RS00120 16437..17459 + 1023 WP_000361404.1 helicase UvrD -
FNA74_RS00125 18003..18911 + 909 WP_032425603.1 HNH endonuclease -
FNA74_RS00130 19097..19447 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..203764 203764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T128730 WP_001044770.1 NZ_CP041514:14400-14816 [Klebsiella michiganensis]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T128730 NZ_CP041514:14400-14816 [Klebsiella michiganensis]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT128730 WP_001261282.1 NZ_CP041514:14173-14403 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT128730 NZ_CP041514:14173-14403 [Klebsiella michiganensis]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References