Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3573..4098 Replicon plasmid unnamed
Accession NZ_CP041514
Organism Klebsiella michiganensis strain KNU07

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag FNA74_RS00030 Protein ID WP_004197633.1
Coordinates 3573..3878 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag FNA74_RS00035 Protein ID WP_006788213.1
Coordinates 3880..4098 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA74_RS30560 236..349 + 114 WP_014343462.1 hypothetical protein -
FNA74_RS00005 478..735 + 258 WP_009310077.1 hypothetical protein -
FNA74_RS00010 793..1572 - 780 WP_023287113.1 site-specific integrase -
FNA74_RS00015 1770..2785 - 1016 Protein_3 hypothetical protein -
FNA74_RS00020 2819..3154 - 336 WP_009309920.1 hypothetical protein -
FNA74_RS00030 3573..3878 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
FNA74_RS00035 3880..4098 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FNA74_RS00050 4695..4925 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
FNA74_RS00055 4922..5338 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
FNA74_RS00060 5379..6257 - 879 WP_032427228.1 restriction endonuclease -
FNA74_RS00065 6919..8195 + 1277 Protein_10 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..203764 203764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T128728 WP_004197633.1 NZ_CP041514:c3878-3573 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T128728 NZ_CP041514:c3878-3573 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT128728 WP_006788213.1 NZ_CP041514:c4098-3880 [Klebsiella michiganensis]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT128728 NZ_CP041514:c4098-3880 [Klebsiella michiganensis]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References