Detailed information of TA system TA128710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 79986..80743
• Replicon: plasmid unnamed
• Accession: NZ_CP041512
• Organism: Shigella boydii strain KCCM 41690

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SN7
• Locus tag: FNA65_RS00570
• Protein ID: WP_000501969.1
• Coordinates: 80258..80743 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: FNA65_RS00565
• Protein ID: WP_011114751.1
• Coordinates: 79986..80270 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA65_RS24850	75423..76061	+	639	WP_000607008.1	type III secretion system effector OspI	-
FNA65_RS00530	76176..76445	+	270	Protein_94	transposase	-
FNA65_RS00535	76533..77761	+	1229	WP_167549346.1	IS3-like element IS2 family transposase	-
FNA65_RS00540	77774..77878	-	105	Protein_96	DUF1472 domain-containing protein	-
FNA65_RS00545	78009..78135	+	127	Protein_97	IS110 family transposase	-
FNA65_RS00565	79986..80270	+	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
FNA65_RS00570	80258..80743	+	486	WP_000501969.1	GNAT family N-acetyltransferase	Toxin
FNA65_RS00575	80784..82376	-	1593	WP_001072392.1	IS66 family transposase	-
FNA65_RS00580	82407..82757	-	351	Protein_102	IS66 family insertion sequence element accessory protein TnpB	-
FNA65_RS00590	82879..84088	+	1210	Protein_103	IS3 family transposase	-
FNA65_RS00595	84125..84304	+	180	Protein_104	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospC4 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospD2	1..108375	108375
	-	inside	IScluster/Tn	-	ospE2 / ipaH1.4 / ospI	52065..84313	32248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17712.54 Da
• Isoelectric Point: 10.1083

>T128710 WP_000501969.1 NZ_CP041512:80258-80743 [Shigella boydii]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T128710 NZ_CP041512:80258-80743 [Shigella boydii]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT128710 WP_011114751.1 NZ_CP041512:79986-80270 [Shigella boydii]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT128710 NZ_CP041512:79986-80270 [Shigella boydii]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: Q31SN7

Antitoxin

• AlphaFold DB: A0A4V1CUJ1