Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 15200..15975 Replicon plasmid unnamed
Accession NZ_CP041512
Organism Shigella boydii strain KCCM 41690

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag FNA65_RS00095 Protein ID WP_000405248.1
Coordinates 15200..15682 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag FNA65_RS00100 Protein ID WP_010921625.1
Coordinates 15673..15975 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA65_RS00075 10704..11651 - 948 WP_005005342.1 omptin family outer membrane protease IcsP -
FNA65_RS00080 13031..13183 - 153 Protein_13 IS3 family transposase -
FNA65_RS00085 13239..13931 + 693 WP_000625263.1 type III secretion system effector cysteine methyltransferase OspZ -
FNA65_RS00090 13955..15031 - 1077 Protein_15 IS3 family transposase -
FNA65_RS00095 15200..15682 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
FNA65_RS00100 15673..15975 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
FNA65_RS00110 16247..16519 + 273 WP_000019158.1 hypothetical protein -
FNA65_RS00120 17927..19021 + 1095 Protein_20 IS91 family transposase -
FNA65_RS00125 19193..19321 + 129 Protein_21 IS5/IS1182 family transposase -
FNA65_RS00135 19470..20924 - 1455 WP_000701110.1 type 3 secretion system effector OspC2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC4 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / ospE2 / ipaH1.4 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospD2 / ospD2 1..108375 108375
- inside IScluster/Tn - ospC4 / ospB / icsP/sopA / nleE / ospC2 440..29058 28618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T128707 WP_000405248.1 NZ_CP041512:c15682-15200 [Shigella boydii]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T128707 NZ_CP041512:c15682-15200 [Shigella boydii]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT128707 WP_010921625.1 NZ_CP041512:c15975-15673 [Shigella boydii]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT128707 NZ_CP041512:c15975-15673 [Shigella boydii]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCTTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References