Detailed information of TA system TA128691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1521493..1522330
• Replicon: chromosome
• Accession: NZ_CP041511
• Organism: Shigella sonnei strain KCCM 41282

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FNA55_RS07390
• Protein ID: WP_000227784.1
• Coordinates: 1521493..1522035 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FNA55_RS07395
• Protein ID: WP_001297137.1
• Coordinates: 1522019..1522330 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNA55_RS07360 (1516557)	1516557..1517048	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FNA55_RS07365 (1517176)	1517176..1518540	-	1365	WP_001000971.1	MFS transporter	-
FNA55_RS07370 (1518659)	1518659..1519356	+	698	WP_237158176.1	IS1-like element IS1A family transposase	-
FNA55_RS07375 (1519725)	1519725..1520123	+	399	Protein_1420	SEL1-like repeat protein	-
FNA55_RS07385 (1520871)	1520871..1521437	+	567	Protein_1422	SEL1-like repeat protein	-
FNA55_RS07390 (1521493)	1521493..1522035	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FNA55_RS07395 (1522019)	1522019..1522330	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FNA55_RS07400 (1522515)	1522515..1523405	-	891	WP_000971327.1	heme o synthase	-
FNA55_RS07405 (1523417)	1523417..1523746	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FNA55_RS07410 (1523746)	1523746..1524360	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FNA55_RS07415 (1524350)	1524350..1526341	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FNA55_RS07420 (1526363)	1526363..1527310	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T128691 WP_000227784.1 NZ_CP041511:c1522035-1521493 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T128691 NZ_CP041511:c1522035-1521493 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT128691 WP_001297137.1 NZ_CP041511:c1522330-1522019 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT128691 NZ_CP041511:c1522330-1522019 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3