Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 820264..820761 Replicon chromosome
Accession NZ_CP041511
Organism Shigella sonnei strain KCCM 41282

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FNA55_RS03990 Protein ID WP_001173343.1
Coordinates 820588..820761 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FNA55_RS03985 Protein ID WP_000398619.1
Coordinates 820264..820560 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNA55_RS03965 (816995) 816995..817714 - 720 WP_000611285.1 two-component system response regulator DcuR -
FNA55_RS03970 (817711) 817711..819342 - 1632 WP_001216495.1 sensor histidine kinase -
FNA55_RS03975 (819523) 819523..819753 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
FNA55_RS03980 (819765) 819765..820037 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
FNA55_RS03985 (820264) 820264..820560 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FNA55_RS03990 (820588) 820588..820761 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FNA55_RS03995 (820880) 820880..822397 - 1518 WP_001295086.1 lysine--tRNA ligase -
FNA55_RS04000 (822634) 822634..824091 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
FNA55_RS04005 (824150) 824150..824407 - 258 Protein_757 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T128689 WP_001173343.1 NZ_CP041511:820588-820761 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T128689 NZ_CP041511:820588-820761 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT128689 WP_000398619.1 NZ_CP041511:820264-820560 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT128689 NZ_CP041511:820264-820560 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References