Detailed information of TA system TA128667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37969..38766
• Replicon: plasmid pYPE3-114k
• Accession: NZ_CP041451
• Organism: Escherichia coli strain YPE3

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: FNP78_RS00255
• Protein ID: WP_172754447.1
• Coordinates: 38263..38766 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: FNP78_RS00250
• Protein ID: WP_016582626.1
• Coordinates: 37969..38238 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP78_RS00225	33291..34631	-	1341	WP_023135655.1	AAA family ATPase	-
FNP78_RS00230	34692..35417	-	726	WP_151164915.1	hypothetical protein	-
FNP78_RS00235	35701..36468	+	768	WP_000342417.1	hypothetical protein	-
FNP78_RS00240	36521..36874	-	354	WP_160378290.1	hypothetical protein	-
FNP78_RS00245	36880..37548	-	669	WP_000161228.1	AAA family ATPase	-
FNP78_RS00250	37969..38238	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
FNP78_RS00255	38263..38766	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
FNP78_RS23840	38794..39000	-	207	WP_023316023.1	hypothetical protein	-
FNP78_RS23845	38951..39202	-	252	WP_000147960.1	hypothetical protein	-
FNP78_RS00265	39204..39896	-	693	WP_151164916.1	hypothetical protein	-
FNP78_RS00270	39910..40233	-	324	WP_032084070.1	hypothetical protein	-
FNP78_RS00275	40308..41090	-	783	WP_151164917.1	receptor-recognizing protein	-
FNP78_RS23850	41157..41810	-	654	WP_191963540.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114322	114322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T128667 WP_172754447.1 NZ_CP041451:38263-38766 [Escherichia coli]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T128667 NZ_CP041451:38263-38766 [Escherichia coli]
ATGTTTTCAGCACAAGCCGTATACGATTTTTCTGGCTTCGACTGCGGCGAACCATCTCTGAATGATTTCCTGCAAAACCG
CCTAGTACAGCAACACAACGGACGTATATTACGCGGCTACCTGCTTCTTACCAAAGATCCGGTGCCGAAAGTTAAGGGTT
TTTACACGCTATCCGGGAGCTGTTTTGCAAGACAGACGCTACCATCGAACACCCAGAAGCGGAAAATCCCCTACGCAGAT
GCACCCAGCGTTACGCTTGGCCGCCTGGCAATAGATATCAGCCTACAGAAACAAGGCTACGGTGAGGTTCTCGTAACGCA
CGCGTTGAAGGTTGTTTATCAGGCTTCTCGCGCAGTAGGCATATACGCTTTGTTTGTGGATGCACTAAACCAAAGCGCAA
TGCAGTTCTATCAGAAGTTTGGATTCATCCCTTTGACGGGGGCGAATGCGAACTCGCTTTTCTACCCCACGAAAAGCATT
GAAGAGCTGTTTGAAACAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT128667 WP_016582626.1 NZ_CP041451:37969-38238 [Escherichia coli]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT128667 NZ_CP041451:37969-38238 [Escherichia coli]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTAAGATTAACTGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACTATTACTCAGTTTATGGTTGCCAGCGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3