Detailed information of TA system TA128666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 31287..31885
• Replicon: plasmid pYPE3-114k
• Accession: NZ_CP041451
• Organism: Escherichia coli strain YPE3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: FNP78_RS00210
• Protein ID: WP_053291281.1
• Coordinates: 31508..31885 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: FNP78_RS00205
• Protein ID: WP_001603498.1
• Coordinates: 31287..31508 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP78_RS00185	26290..26841	+	552	WP_151164913.1	fimbrial protein YehD	-
FNP78_RS00190	26897..27577	+	681	WP_032187672.1	fimbrial assembly chaperone	-
FNP78_RS00195	27595..30081	+	2487	WP_096261919.1	fimbria/pilus outer membrane usher protein	-
FNP78_RS00200	30092..31102	+	1011	WP_151164914.1	type 1 fimbrial protein	-
FNP78_RS00205	31287..31508	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FNP78_RS00210	31508..31885	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FNP78_RS00220	32019..33134	-	1116	WP_171764655.1	DNA primase	-
FNP78_RS00225	33291..34631	-	1341	WP_023135655.1	AAA family ATPase	-
FNP78_RS00230	34692..35417	-	726	WP_151164915.1	hypothetical protein	-
FNP78_RS00235	35701..36468	+	768	WP_000342417.1	hypothetical protein	-
FNP78_RS00240	36521..36874	-	354	WP_160378290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114322	114322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T128666 WP_053291281.1 NZ_CP041451:31508-31885 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T128666 NZ_CP041451:31508-31885 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT128666 WP_001603498.1 NZ_CP041451:31287-31508 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT128666 NZ_CP041451:31287-31508 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0