Detailed information of TA system TA128653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2448812..2449338
• Replicon: chromosome
• Accession: NZ_CP041448
• Organism: Escherichia coli strain YPE10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FNP79_RS12400
• Protein ID: WP_000323025.1
• Coordinates: 2448812..2449099 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FNP79_RS12405
• Protein ID: WP_000534858.1
• Coordinates: 2449099..2449338 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNP79_RS12355	2443844..2444059	-	216	WP_000839590.1	phage lysis protein EssD	-
FNP79_RS12360	2444813..2445028	-	216	WP_000066484.1	cold shock-like protein CspB	-
FNP79_RS12365	2445329..2445541	+	213	WP_000087756.1	cold shock-like protein CspF	-
FNP79_RS12370	2445596..2445685	+	90	WP_120795389.1	hypothetical protein	-
FNP79_RS12375	2445963..2446715	-	753	WP_001047135.1	antitermination protein	-
FNP79_RS12380	2446712..2447770	-	1059	WP_064670548.1	DUF968 domain-containing protein	-
FNP79_RS12385	2447772..2448050	-	279	WP_012304870.1	hypothetical protein	-
FNP79_RS12390	2448117..2448368	-	252	WP_000980994.1	hypothetical protein	-
FNP79_RS12395	2448585..2448740	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
FNP79_RS12400	2448812..2449099	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FNP79_RS12405	2449099..2449338	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FNP79_RS12410	2449363..2449668	+	306	WP_001326990.1	hypothetical protein	-
FNP79_RS12415	2449871..2450203	+	333	WP_001301033.1	protein FlxA	-
FNP79_RS12420	2450681..2451843	+	1163	WP_089566769.1	IS3-like element IS3 family transposase	-
FNP79_RS12425	2451901..2453214	-	1314	WP_000589005.1	ISNCY family transposase	-
FNP79_RS12435	2453694..2453882	+	189	WP_000854559.1	cell division inhibition protein DicB	-
FNP79_RS12440	2453879..2454070	+	192	WP_001083297.1	lysis protein YdfD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2433838..2459480	25642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T128653 WP_000323025.1 NZ_CP041448:c2449099-2448812 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T128653 NZ_CP041448:c2449099-2448812 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT128653 WP_000534858.1 NZ_CP041448:c2449338-2449099 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT128653 NZ_CP041448:c2449338-2449099 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8