Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1214355..1214973 Replicon chromosome
Accession NZ_CP041448
Organism Escherichia coli strain YPE10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FNP79_RS06330 Protein ID WP_001291435.1
Coordinates 1214355..1214573 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FNP79_RS06335 Protein ID WP_000344800.1
Coordinates 1214599..1214973 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP79_RS06295 1209644..1210216 + 573 WP_000779831.1 YbaY family lipoprotein -
FNP79_RS06300 1210247..1210558 - 312 WP_000409911.1 MGMT family protein -
FNP79_RS06310 1210937..1211290 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FNP79_RS06315 1211332..1212882 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FNP79_RS06320 1213046..1213516 - 471 WP_000136192.1 YlaC family protein -
FNP79_RS06325 1213632..1214183 - 552 WP_000102564.1 maltose O-acetyltransferase -
FNP79_RS06330 1214355..1214573 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FNP79_RS06335 1214599..1214973 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FNP79_RS06340 1215519..1218668 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FNP79_RS06345 1218691..1219884 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128649 WP_001291435.1 NZ_CP041448:c1214573-1214355 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128649 NZ_CP041448:c1214573-1214355 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT128649 WP_000344800.1 NZ_CP041448:c1214973-1214599 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128649 NZ_CP041448:c1214973-1214599 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References