Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 36578..37100 Replicon plasmid pYPE10-78k
Accession NZ_CP041444
Organism Escherichia coli strain YPE10

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag FNP79_RS00235 Protein ID WP_000220560.1
Coordinates 36578..36859 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FNP79_RS00240 Protein ID WP_000121743.1
Coordinates 36849..37100 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP79_RS00200 32847..33119 + 273 Protein_36 IS1 family transposase -
FNP79_RS00205 33139..33336 - 198 WP_000844102.1 hypothetical protein -
FNP79_RS00210 33435..34403 - 969 WP_000654802.1 IS5-like element IS903B family transposase -
FNP79_RS00215 34425..34895 - 471 WP_094320909.1 restriction endonuclease -
FNP79_RS00220 35104..35415 - 312 WP_001452736.1 hypothetical protein -
FNP79_RS00225 35451..35765 - 315 WP_000734776.1 IncN plasmid KikA protein -
FNP79_RS00230 35806..36503 - 698 Protein_42 IS1 family transposase -
FNP79_RS00235 36578..36859 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNP79_RS00240 36849..37100 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FNP79_RS00255 38334..39170 + 837 WP_001050931.1 replication initiation protein -
FNP79_RS00260 39210..39656 + 447 Protein_46 hypothetical protein -
FNP79_RS00265 39724..40149 + 426 WP_000202942.1 hypothetical protein -
FNP79_RS00270 40146..41123 - 978 WP_024245156.1 hypothetical protein -
FNP79_RS00275 41148..41429 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / floR / dfrA12 / aadA2 / tet(M) / qnrS1 / blaTEM-1B / tet(A) - 1..78706 78706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T128643 WP_000220560.1 NZ_CP041444:c36859-36578 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T128643 NZ_CP041444:c36859-36578 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT128643 WP_000121743.1 NZ_CP041444:c37100-36849 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT128643 NZ_CP041444:c37100-36849 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References