Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 64271..64793 Replicon plasmid pYPE12-101k-tetX4
Accession NZ_CP041443
Organism Escherichia coli strain YPE12

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag FNP80_RS25120 Protein ID WP_000220560.1
Coordinates 64512..64793 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FNP80_RS25115 Protein ID WP_000121743.1
Coordinates 64271..64522 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP80_RS25080 59945..60226 + 282 WP_001185482.1 hypothetical protein -
FNP80_RS25085 60251..61228 + 978 WP_024245156.1 hypothetical protein -
FNP80_RS25090 61225..61650 - 426 WP_000202942.1 hypothetical protein -
FNP80_RS25095 61718..62164 - 447 Protein_77 hypothetical protein -
FNP80_RS25100 62204..63040 - 837 WP_001050931.1 replication initiation protein -
FNP80_RS25115 64271..64522 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FNP80_RS25120 64512..64793 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNP80_RS25125 64868..65565 + 698 Protein_81 IS1 family transposase -
FNP80_RS25135 65711..65968 + 258 WP_105278499.1 hypothetical protein -
FNP80_RS25140 66005..66969 - 965 Protein_83 IS5 family transposase -
FNP80_RS25145 67004..67279 + 276 Protein_84 peptidoglycan DD-metalloendopeptidase family protein -
FNP80_RS25150 67313..67510 - 198 WP_000843497.1 DUF2933 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / tet(M) / blaTEM-1B / tet(A) / floR / tet(X4) / dfrA5 / sul3 / mef(B) - 1..101987 101987


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T128642 WP_000220560.1 NZ_CP041443:64512-64793 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T128642 NZ_CP041443:64512-64793 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT128642 WP_000121743.1 NZ_CP041443:64271-64522 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT128642 NZ_CP041443:64271-64522 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References