Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 97831..98353 Replicon plasmid pYPE12-106k
Accession NZ_CP041439
Organism Escherichia coli strain YPE12

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag FNP80_RS00695 Protein ID WP_000220561.1
Coordinates 98072..98353 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FNP80_RS00690 Protein ID WP_000121743.1
Coordinates 97831..98082 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNP80_RS00665 93954..94376 - 423 WP_179292036.1 DNA distortion polypeptide 3 -
FNP80_RS00670 94444..94890 - 447 WP_001074384.1 hypothetical protein -
FNP80_RS00675 94894..95736 - 843 WP_063116919.1 replication initiation protein -
FNP80_RS00680 95757..96596 - 840 WP_024129959.1 replication initiation protein -
FNP80_RS00690 97831..98082 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FNP80_RS00695 98072..98353 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNP80_RS00700 98592..98684 - 93 Protein_119 IS6 family transposase -
FNP80_RS00705 98850..100049 - 1200 WP_000948429.1 IS91 family transposase -
FNP80_RS00710 100059..100247 - 189 WP_000957857.1 hypothetical protein -
FNP80_RS00715 100339..100875 + 537 WP_064441864.1 recombinase family protein -
FNP80_RS00720 101058..101918 + 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
FNP80_RS00725 102088..102843 + 756 WP_012372818.1 16S rRNA (guanine(1405)-N(7))-methyltransferase RmtB1 -
FNP80_RS00730 102924..103205 - 282 WP_015059004.1 sodium/hydrogen exchanger -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / blaTEM-1B / rmtB - 1..106630 106630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T128617 WP_000220561.1 NZ_CP041439:98072-98353 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T128617 NZ_CP041439:98072-98353 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT128617 WP_000121743.1 NZ_CP041439:97831-98082 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT128617 NZ_CP041439:97831-98082 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References