Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 23607..24132 Replicon plasmid pSTEC005
Accession NZ_CP041438
Organism Escherichia coli strain STEC005

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FNE80_RS24030 Protein ID WP_001159871.1
Coordinates 23827..24132 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FNE80_RS24025 Protein ID WP_000813634.1
Coordinates 23607..23825 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE80_RS24000 18927..19508 - 582 WP_096934554.1 acetyltransferase -
FNE80_RS24005 19486..20682 - 1197 WP_161619135.1 glycosyltransferase family 2 protein -
FNE80_RS24010 20693..21358 - 666 WP_096934566.1 PIG-L family deacetylase -
FNE80_RS24015 22449..22652 + 204 Protein_23 transposase -
FNE80_RS24020 22642..23067 + 426 Protein_24 hypothetical protein -
FNE80_RS24025 23607..23825 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FNE80_RS24030 23827..24132 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FNE80_RS24035 24133..24942 + 810 WP_161619134.1 site-specific integrase -
FNE80_RS24040 25080..25355 - 276 WP_000239529.1 hypothetical protein -
FNE80_RS24045 25349..25993 - 645 WP_000633911.1 AAA family ATPase -
FNE80_RS24050 26222..27193 + 972 WP_001103694.1 hypothetical protein -
FNE80_RS24055 27198..27590 + 393 WP_000340833.1 plasmid partitioning/stability family protein -
FNE80_RS24065 27595..28865 - 1271 Protein_32 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD 1..89435 89435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T128606 WP_001159871.1 NZ_CP041438:23827-24132 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T128606 NZ_CP041438:23827-24132 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT128606 WP_000813634.1 NZ_CP041438:23607-23825 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT128606 NZ_CP041438:23607-23825 [Escherichia coli]
ATGAAACAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References