Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3680384..3681002 Replicon chromosome
Accession NZ_CP041437
Organism Escherichia coli strain STEC005

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FNE80_RS17995 Protein ID WP_001291435.1
Coordinates 3680784..3681002 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FNE80_RS17990 Protein ID WP_000344800.1
Coordinates 3680384..3680758 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE80_RS17980 3675473..3676666 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FNE80_RS17985 3676689..3679838 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FNE80_RS17990 3680384..3680758 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FNE80_RS17995 3680784..3681002 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FNE80_RS18000 3681173..3681724 + 552 WP_000102578.1 maltose O-acetyltransferase -
FNE80_RS18005 3681840..3682310 + 471 WP_000136192.1 YlaC family protein -
FNE80_RS18010 3682474..3684024 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FNE80_RS18015 3684066..3684419 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FNE80_RS18025 3684798..3685109 + 312 WP_000409911.1 MGMT family protein -
FNE80_RS18030 3685140..3685712 - 573 WP_161620945.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128600 WP_001291435.1 NZ_CP041437:3680784-3681002 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128600 NZ_CP041437:3680784-3681002 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT128600 WP_000344800.1 NZ_CP041437:3680384-3680758 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128600 NZ_CP041437:3680384-3680758 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References