Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1369309..1369934 Replicon chromosome
Accession NZ_CP041437
Organism Escherichia coli strain STEC005

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag FNE80_RS06580 Protein ID WP_000911329.1
Coordinates 1369536..1369934 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FNE80_RS06575 Protein ID WP_000450524.1
Coordinates 1369309..1369536 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE80_RS06550 1365111..1365581 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FNE80_RS06555 1365581..1366153 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FNE80_RS06560 1366299..1367177 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
FNE80_RS06565 1367194..1368228 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FNE80_RS06570 1368441..1369154 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FNE80_RS06575 1369309..1369536 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FNE80_RS06580 1369536..1369934 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
FNE80_RS06585 1370081..1370944 + 864 WP_001267498.1 neutral zinc metallopeptidase -
FNE80_RS06590 1370959..1372974 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
FNE80_RS06595 1373048..1373746 + 699 WP_000679823.1 esterase -
FNE80_RS06600 1373856..1374056 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T128589 WP_000911329.1 NZ_CP041437:1369536-1369934 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T128589 NZ_CP041437:1369536-1369934 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT128589 WP_000450524.1 NZ_CP041437:1369309-1369536 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT128589 NZ_CP041437:1369309-1369536 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References