128583

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58439..58964 Replicon plasmid pSTEC309
Accession NZ_CP041436
Organism Escherichia coli strain STEC309

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FNE81_RS24290 Protein ID WP_001159871.1
Coordinates 58439..58744 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FNE81_RS24295 Protein ID WP_000813634.1
Coordinates 58746..58964 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE81_RS24255 53706..54976 + 1271 Protein_67 translesion error-prone DNA polymerase V subunit UmuC -
FNE81_RS24265 54981..55373 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
FNE81_RS24270 55378..56349 - 972 WP_001103694.1 hypothetical protein -
FNE81_RS24275 56578..57222 + 645 WP_000633911.1 AAA family ATPase -
FNE81_RS24280 57216..57491 + 276 WP_000239529.1 hypothetical protein -
FNE81_RS24285 57629..58438 - 810 WP_161619134.1 site-specific integrase -
FNE81_RS24290 58439..58744 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FNE81_RS24295 58746..58964 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FNE81_RS24300 59919..60122 - 204 Protein_75 transposase -
FNE81_RS24305 61216..61881 + 666 WP_096934566.1 PIG-L family deacetylase -
FNE81_RS24310 61892..63088 + 1197 WP_161619135.1 glycosyltransferase family 2 protein -
FNE81_RS24315 63066..63647 + 582 WP_096934554.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD 1..88727 88727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T128583 WP_001159871.1 NZ_CP041436:c58744-58439 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T128583 NZ_CP041436:c58744-58439 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT128583 WP_000813634.1 NZ_CP041436:c58964-58746 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT128583 NZ_CP041436:c58964-58746 [Escherichia coli]
ATGAAACAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References