Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58439..58964 Replicon plasmid pSTEC309
Accession NZ_CP041436
Organism Escherichia coli strain STEC309

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FNE81_RS24290 Protein ID WP_001159871.1
Coordinates 58439..58744 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FNE81_RS24295 Protein ID WP_000813634.1
Coordinates 58746..58964 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE81_RS24255 53706..54976 + 1271 Protein_67 translesion error-prone DNA polymerase V subunit UmuC -
FNE81_RS24265 54981..55373 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
FNE81_RS24270 55378..56349 - 972 WP_001103694.1 hypothetical protein -
FNE81_RS24275 56578..57222 + 645 WP_000633911.1 AAA family ATPase -
FNE81_RS24280 57216..57491 + 276 WP_000239529.1 hypothetical protein -
FNE81_RS24285 57629..58438 - 810 WP_161619134.1 site-specific integrase -
FNE81_RS24290 58439..58744 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FNE81_RS24295 58746..58964 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FNE81_RS24300 59919..60122 - 204 Protein_75 transposase -
FNE81_RS24305 61216..61881 + 666 WP_096934566.1 PIG-L family deacetylase -
FNE81_RS24310 61892..63088 + 1197 WP_161619135.1 glycosyltransferase family 2 protein -
FNE81_RS24315 63066..63647 + 582 WP_096934554.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD 1..88727 88727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T128583 WP_001159871.1 NZ_CP041436:c58744-58439 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T128583 NZ_CP041436:c58744-58439 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT128583 WP_000813634.1 NZ_CP041436:c58964-58746 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT128583 NZ_CP041436:c58964-58746 [Escherichia coli]
ATGAAACAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References