128582

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 12966..13591 Replicon plasmid pSTEC309
Accession NZ_CP041436
Organism Escherichia coli strain STEC309

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag FNE81_RS23975 Protein ID WP_000911315.1
Coordinates 13193..13591 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag FNE81_RS23970 Protein ID WP_000450532.1
Coordinates 12966..13193 (+) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE81_RS23970 12966..13193 + 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin
FNE81_RS23975 13193..13591 + 399 WP_000911315.1 tRNA(fMet)-specific endonuclease VapC Toxin
FNE81_RS23980 13600..15753 - 2154 WP_161619128.1 type IV conjugative transfer system coupling protein TraD -
FNE81_RS23985 16006..16737 - 732 WP_000850422.1 complement resistance protein TraT -
FNE81_RS23990 16762..17283 - 522 WP_000632670.1 conjugal transfer entry exclusion protein TraS -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD 1..88727 88727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-125)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 14871.17 Da        Isoelectric Point: 8.5264

>T128582 WP_000911315.1 NZ_CP041436:13193-13591 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T128582 NZ_CP041436:13193-13591 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGCACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT128582 WP_000450532.1 NZ_CP041436:12966-13193 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT128582 NZ_CP041436:12966-13193 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References