Detailed information of TA system TA128572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2490492..2491130
• Replicon: chromosome
• Accession: NZ_CP041435
• Organism: Escherichia coli strain STEC309

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FNE81_RS11990
• Protein ID: WP_000813794.1
• Coordinates: 2490954..2491130 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FNE81_RS11985
• Protein ID: WP_001270286.1
• Coordinates: 2490492..2490908 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE81_RS11965	2485644..2486585	-	942	WP_001251319.1	ABC transporter permease	-
FNE81_RS11970	2486586..2487599	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FNE81_RS11975	2487617..2488762	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
FNE81_RS11980	2489007..2490413	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
FNE81_RS11985	2490492..2490908	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FNE81_RS11990	2490954..2491130	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FNE81_RS11995	2491352..2491582	+	231	WP_000494244.1	YncJ family protein	-
FNE81_RS12000	2491674..2493635	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FNE81_RS12005	2493708..2494244	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
FNE81_RS12010	2494297..2495511	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2495551..2496816	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T128572 WP_000813794.1 NZ_CP041435:c2491130-2490954 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T128572 NZ_CP041435:c2491130-2490954 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT128572 WP_001270286.1 NZ_CP041435:c2490908-2490492 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT128572 NZ_CP041435:c2490908-2490492 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB