Detailed information of TA system TA128529

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2531880..2532715
• Replicon: chromosome
• Accession: NZ_CP041433
• Organism: Escherichia coli strain STEC313

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: V0Z9U3
• Locus tag: FNE82_RS12425
• Protein ID: WP_000854750.1
• Coordinates: 2532338..2532715 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J7L0S7
• Locus tag: FNE82_RS12420
• Protein ID: WP_001278231.1
• Coordinates: 2531880..2532248 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE82_RS12385	2527697..2528377	+	681	WP_001282919.1	WYL domain-containing protein	-
FNE82_RS12390	2528525..2529202	+	678	WP_001097305.1	hypothetical protein	-
FNE82_RS12395	2529208..2529441	+	234	WP_001278287.1	DUF905 family protein	-
FNE82_RS12400	2529540..2530358	+	819	WP_001234621.1	DUF945 domain-containing protein	-
FNE82_RS12405	2530450..2530935	+	486	WP_061356738.1	antirestriction protein	-
FNE82_RS12410	2530951..2531427	+	477	WP_061356739.1	RadC family protein	-
FNE82_RS12415	2531496..2531717	+	222	WP_000692353.1	DUF987 domain-containing protein	-
FNE82_RS12420	2531880..2532248	+	369	WP_001278231.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNE82_RS12425	2532338..2532715	+	378	WP_000854750.1	TA system toxin CbtA family protein	Toxin
FNE82_RS12430	2532712..2533200	+	489	WP_000761685.1	hypothetical protein	-
FNE82_RS12435	2533212..2533409	+	198	WP_000839280.1	DUF957 domain-containing protein	-
FNE82_RS25420	2533494..2533640	+	147	WP_001290178.1	hypothetical protein	-
FNE82_RS12445	2534292..2537183	+	2892	WP_001395333.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13919.90 Da
• Isoelectric Point: 8.2905

>T128529 WP_000854750.1 NZ_CP041433:2532338-2532715 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128529 NZ_CP041433:2532338-2532715 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT128529 WP_001278231.1 NZ_CP041433:2531880-2532248 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLEIMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128529 NZ_CP041433:2531880-2532248 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAATCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: V0Z9U3

Antitoxin

• AlphaFold DB: A0A2J7L0S7