Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4719468..4719965 Replicon chromosome
Accession NZ_CP041431
Organism Escherichia coli strain STEC316

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FNE83_RS23340 Protein ID WP_001173343.1
Coordinates 4719468..4719641 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FNE83_RS23345 Protein ID WP_000398619.1
Coordinates 4719669..4719965 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE83_RS23330 4716138..4717595 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
FNE83_RS23335 4717832..4719349 + 1518 WP_001295074.1 lysine--tRNA ligase -
FNE83_RS23340 4719468..4719641 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FNE83_RS23345 4719669..4719965 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FNE83_RS23350 4720192..4720464 - 273 WP_000405647.1 N-acetyltransferase -
FNE83_RS23355 4720476..4720706 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
FNE83_RS23360 4720887..4722518 + 1632 WP_001216486.1 sensor histidine kinase -
FNE83_RS23365 4722515..4723234 + 720 WP_161620808.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T128509 WP_001173343.1 NZ_CP041431:c4719641-4719468 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T128509 NZ_CP041431:c4719641-4719468 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT128509 WP_000398619.1 NZ_CP041431:c4719965-4719669 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT128509 NZ_CP041431:c4719965-4719669 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References