Detailed information of TA system TA128500

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2921565..2922400
• Replicon: chromosome
• Accession: NZ_CP041431
• Organism: Escherichia coli strain STEC316

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: FNE83_RS14710
• Protein ID: WP_000854752.1
• Coordinates: 2922023..2922400 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: FNE83_RS14705
• Protein ID: WP_001278232.1
• Coordinates: 2921565..2921933 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE83_RS14675	2918401..2918856	+	456	WP_000581504.1	hypothetical protein	-
FNE83_RS14680	2918935..2919168	+	234	WP_001119729.1	DUF905 family protein	-
FNE83_RS14685	2919268..2920086	+	819	WP_001234620.1	DUF945 domain-containing protein	-
FNE83_RS14690	2920141..2920626	+	486	WP_000849582.1	antirestriction protein	-
FNE83_RS14695	2920642..2921118	+	477	WP_001186726.1	RadC family protein	-
FNE83_RS14700	2921181..2921402	+	222	WP_000692329.1	DUF987 domain-containing protein	-
FNE83_RS14705	2921565..2921933	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FNE83_RS14710	2922023..2922400	+	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
FNE83_RS14715	2922397..2922600	+	204	WP_161620747.1	hypothetical protein	-
FNE83_RS14720	2922613..2922726	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FNE83_RS26635	2923542..2923913	+	372	WP_001295631.1	IS110 family transposase	-
FNE83_RS14735	2924367..2924549	+	183	WP_024167529.1	ethanolamine utilization protein	-
FNE83_RS14740	2924650..2924979	-	330	WP_000450409.1	DUF496 family protein	-
FNE83_RS14745	2925151..2926209	-	1059	WP_001200892.1	FUSC family protein	-
FNE83_RS14750	2926407..2926880	-	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T128500 WP_000854752.1 NZ_CP041431:2922023-2922400 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T128500 NZ_CP041431:2922023-2922400 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT128500 WP_001278232.1 NZ_CP041431:2921565-2921933 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128500 NZ_CP041431:2921565-2921933 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72