Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3078363..3078981 Replicon chromosome
Accession NZ_CP041429
Organism Escherichia coli strain STEC367

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FNE84_RS14890 Protein ID WP_001291435.1
Coordinates 3078363..3078581 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FNE84_RS14895 Protein ID WP_000344800.1
Coordinates 3078607..3078981 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE84_RS14855 3073652..3074224 + 573 WP_000779826.1 lipoprotein -
FNE84_RS14860 3074255..3074566 - 312 WP_000409912.1 MGMT family protein -
FNE84_RS14870 3074945..3075298 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FNE84_RS14875 3075340..3076890 - 1551 WP_001352692.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FNE84_RS14880 3077054..3077524 - 471 WP_000136192.1 YlaC family protein -
FNE84_RS14885 3077640..3078191 - 552 WP_000102568.1 maltose O-acetyltransferase -
FNE84_RS14890 3078363..3078581 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FNE84_RS14895 3078607..3078981 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FNE84_RS14900 3079527..3082676 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FNE84_RS14905 3082699..3083892 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128451 WP_001291435.1 NZ_CP041429:c3078581-3078363 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128451 NZ_CP041429:c3078581-3078363 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT128451 WP_000344800.1 NZ_CP041429:c3078981-3078607 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128451 NZ_CP041429:c3078981-3078607 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References