Detailed information of TA system TA128448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2535733..2536309
• Replicon: chromosome
• Accession: NZ_CP041429
• Organism: Escherichia coli strain STEC367

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FNE84_RS12365
• Protein ID: WP_161623821.1
• Coordinates: 2535733..2536020 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A080I7H7
• Locus tag: FNE84_RS12370
• Protein ID: WP_000063160.1
• Coordinates: 2536007..2536309 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE84_RS12350	2531971..2533251	-	1281	WP_001037390.1	DUF445 domain-containing protein	-
FNE84_RS12355	2533292..2534524	-	1233	WP_001137031.1	multidrug efflux MFS transporter MdtM	-
FNE84_RS12360	2534838..2535534	+	697	Protein_2417	IS1 family transposase	-
FNE84_RS12365	2535733..2536020	+	288	WP_161623821.1	BrnT family toxin	Toxin
FNE84_RS12370	2536007..2536309	+	303	WP_000063160.1	BrnA antitoxin family protein	Antitoxin
FNE84_RS12375	2536343..2537299	-	957	WP_001297640.1	GTPase	-
FNE84_RS12380	2537310..2537513	-	204	WP_000467859.1	YbdD/YjiX family protein	-
FNE84_RS12385	2537563..2539713	-	2151	WP_001340937.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2535325..2535534	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11326.79 Da
• Isoelectric Point: 6.8821

>T128448 WP_161623821.1 NZ_CP041429:2535733-2536020 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDQQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T128448 NZ_CP041429:2535733-2536020 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCAACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11552.01 Da
• Isoelectric Point: 9.4646

>AT128448 WP_000063160.1 NZ_CP041429:2536007-2536309 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT128448 NZ_CP041429:2536007-2536309 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A080I7H7