Detailed information of TA system TA128447

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2497693..2498528
• Replicon: chromosome
• Accession: NZ_CP041429
• Organism: Escherichia coli strain STEC367

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: V0Z9U3
• Locus tag: FNE84_RS12190
• Protein ID: WP_000854750.1
• Coordinates: 2498151..2498528 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J7L0S7
• Locus tag: FNE84_RS12185
• Protein ID: WP_001278231.1
• Coordinates: 2497693..2498061 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE84_RS12150	2493492..2494172	+	681	WP_001282919.1	WYL domain-containing protein	-
FNE84_RS12155	2494320..2494997	+	678	WP_001097305.1	hypothetical protein	-
FNE84_RS12160	2495003..2495236	+	234	WP_001278287.1	DUF905 family protein	-
FNE84_RS12165	2495335..2496153	+	819	WP_001234615.1	DUF945 domain-containing protein	-
FNE84_RS12170	2496245..2496730	+	486	WP_000206658.1	antirestriction protein	-
FNE84_RS12175	2496746..2497222	+	477	WP_001367707.1	RadC family protein	-
FNE84_RS12180	2497309..2497530	+	222	WP_000691819.1	DUF987 domain-containing protein	-
FNE84_RS12185	2497693..2498061	+	369	WP_001278231.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNE84_RS12190	2498151..2498528	+	378	WP_000854750.1	TA system toxin CbtA family protein	Toxin
FNE84_RS12195	2498525..2499013	+	489	WP_000761685.1	hypothetical protein	-
FNE84_RS12200	2499025..2499222	+	198	WP_000839280.1	DUF957 domain-containing protein	-
FNE84_RS25335	2499307..2499453	+	147	WP_001290178.1	hypothetical protein	-
FNE84_RS12210	2500105..2502996	+	2892	WP_001395333.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13919.90 Da
• Isoelectric Point: 8.2905

>T128447 WP_000854750.1 NZ_CP041429:2498151-2498528 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128447 NZ_CP041429:2498151-2498528 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT128447 WP_001278231.1 NZ_CP041429:2497693-2498061 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLEIMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128447 NZ_CP041429:2497693-2498061 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAATCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: V0Z9U3

Antitoxin

• AlphaFold DB: A0A2J7L0S7