Detailed information of TA system TA128444

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1990005..1990840
• Replicon: chromosome
• Accession: NZ_CP041429
• Organism: Escherichia coli strain STEC367

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: FNE84_RS09500
• Protein ID: WP_000854752.1
• Coordinates: 1990005..1990382 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: FNE84_RS09505
• Protein ID: WP_001278232.1
• Coordinates: 1990472..1990840 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE84_RS09460	1985525..1985998	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
FNE84_RS09465	1986196..1987254	+	1059	WP_001200892.1	FUSC family protein	-
FNE84_RS09470	1987426..1987755	+	330	WP_000450409.1	DUF496 family protein	-
FNE84_RS09475	1987856..1988038	-	183	WP_024167529.1	ethanolamine utilization protein	-
FNE84_RS25460	1988492..1988863	-	372	WP_001295631.1	IS110 family transposase	-
FNE84_RS09490	1989679..1989792	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FNE84_RS09495	1989805..1990008	-	204	WP_161620747.1	hypothetical protein	-
FNE84_RS09500	1990005..1990382	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
FNE84_RS09505	1990472..1990840	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FNE84_RS09510	1991003..1991224	-	222	WP_000692329.1	DUF987 domain-containing protein	-
FNE84_RS09515	1991287..1991763	-	477	WP_001186726.1	RadC family protein	-
FNE84_RS09520	1991779..1992264	-	486	WP_000849582.1	antirestriction protein	-
FNE84_RS09525	1992319..1993137	-	819	WP_001234620.1	DUF945 domain-containing protein	-
FNE84_RS09530	1993237..1993470	-	234	WP_001119729.1	DUF905 family protein	-
FNE84_RS09535	1993549..1994004	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T128444 WP_000854752.1 NZ_CP041429:c1990382-1990005 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T128444 NZ_CP041429:c1990382-1990005 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT128444 WP_001278232.1 NZ_CP041429:c1990840-1990472 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128444 NZ_CP041429:c1990840-1990472 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72