Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1037426..1038080 Replicon chromosome
Accession NZ_CP041429
Organism Escherichia coli strain STEC367

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FNE84_RS05015 Protein ID WP_000244781.1
Coordinates 1037673..1038080 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FNE84_RS05010 Protein ID WP_000354046.1
Coordinates 1037426..1037692 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE84_RS04985 1032595..1033338 + 744 WP_000951964.1 SDR family oxidoreductase -
FNE84_RS04990 1033395..1034828 - 1434 WP_024186065.1 6-phospho-beta-glucosidase BglA -
FNE84_RS04995 1034873..1035184 + 312 WP_047082078.1 N(4)-acetylcytidine aminohydrolase -
FNE84_RS05000 1035348..1036007 + 660 WP_000250274.1 hemolysin III family protein -
FNE84_RS05005 1036203..1037183 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
FNE84_RS05010 1037426..1037692 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FNE84_RS05015 1037673..1038080 + 408 WP_000244781.1 protein YgfX Toxin
FNE84_RS05020 1038120..1038641 - 522 WP_001055874.1 flavodoxin FldB -
FNE84_RS05025 1038753..1039649 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FNE84_RS05030 1039674..1040384 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FNE84_RS05035 1040390..1042123 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T128440 WP_000244781.1 NZ_CP041429:1037673-1038080 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T128440 NZ_CP041429:1037673-1038080 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT128440 WP_000354046.1 NZ_CP041429:1037426-1037692 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT128440 NZ_CP041429:1037426-1037692 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References