Detailed information of TA system TA128435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 61145..61692
• Replicon: plasmid pSTEC388_3
• Accession: NZ_CP041428
• Organism: Escherichia coli strain STEC388

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: FNE85_RS25650
• Protein ID: WP_000969996.1
• Coordinates: 61411..61692 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2I6FMZ7
• Locus tag: FNE85_RS25645
• Protein ID: WP_000079930.1
• Coordinates: 61145..61414 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE85_RS25610	56297..56974	-	678	WP_096101123.1	IS66-like element accessory protein TnpA	-
FNE85_RS25615	57232..57693	+	462	WP_001233868.1	thermonuclease family protein	-
FNE85_RS25620	57739..57948	+	210	WP_001610317.1	hemolysin expression modulator Hha	-
FNE85_RS25625	57986..58576	+	591	WP_161623805.1	DUF2726 domain-containing protein	-
FNE85_RS25630	58816..59070	+	255	WP_000083830.1	replication regulatory protein RepA	-
FNE85_RS25635	59307..59381	+	75	WP_001375168.1	RepA leader peptide Tap	-
FNE85_RS25640	59374..60231	+	858	WP_024236927.1	incFII family plasmid replication initiator RepA	-
FNE85_RS25645	61145..61414	+	270	WP_000079930.1	hypothetical protein	Antitoxin
FNE85_RS25650	61411..61692	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNE85_RS25960	61727..61981	+	255	Protein_77	3'-5' exonuclease	-
FNE85_RS25655	62404..63162	+	759	WP_001258298.1	substrate-binding domain-containing protein	-
FNE85_RS25660	63448..63870	+	423	WP_000202419.1	DUF4238 domain-containing protein	-
FNE85_RS25665	63864..64286	+	423	WP_000883346.1	hypothetical protein	-
FNE85_RS25670	64557..64661	+	105	Protein_81	type II toxin-antitoxin system RelE/ParE family toxin	-
FNE85_RS25675	64777..65127	-	351	WP_000361383.1	plasmid partitioning/stability family protein	-
FNE85_RS25680	65130..66092	-	963	WP_000959875.1	plasmid segregation protein ParM	-
FNE85_RS25685	66239..66532	+	294	WP_032177002.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa	1..67626	67626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T128435 WP_000969996.1 NZ_CP041428:61411-61692 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T128435 NZ_CP041428:61411-61692 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2055

>AT128435 WP_000079930.1 NZ_CP041428:61145-61414 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT128435 NZ_CP041428:61145-61414 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2I6FMZ7