Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 26683..27218 Replicon plasmid pSTEC388_2
Accession NZ_CP041427
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag FNE85_RS25025 Protein ID WP_001270417.1
Coordinates 26931..27218 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag FNE85_RS25020 Protein ID WP_001132895.1
Coordinates 26683..26934 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS24990 21740..22051 - 312 WP_001309256.1 hypothetical protein -
FNE85_RS24995 22115..22471 - 357 WP_178103022.1 DUF1281 domain-containing protein -
FNE85_RS25000 22481..22624 - 144 WP_000270807.1 DUF1281 domain-containing protein -
FNE85_RS25005 22791..23568 - 778 Protein_34 hypothetical protein -
FNE85_RS25010 23802..24764 - 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
FNE85_RS25015 24761..25966 - 1206 WP_000817635.1 AAA family ATPase -
FNE85_RS25925 26340..26510 + 171 WP_170938683.1 hypothetical protein -
FNE85_RS25020 26683..26934 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FNE85_RS25025 26931..27218 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FNE85_RS25030 27217..27465 + 249 Protein_40 transposase -
FNE85_RS25035 28145..29122 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
FNE85_RS25040 29407..30147 - 741 WP_096095888.1 tyrosine-type recombinase/integrase -
FNE85_RS25045 30268..30447 - 180 WP_170938682.1 serine/threonine protein kinase -
FNE85_RS25050 30484..31278 - 795 Protein_44 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - estIa 1..67518 67518


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T128434 WP_001270417.1 NZ_CP041427:26931-27218 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T128434 NZ_CP041427:26931-27218 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT128434 WP_001132895.1 NZ_CP041427:26683-26934 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT128434 NZ_CP041427:26683-26934 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References