Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 12510..13308 Replicon plasmid pSTEC388_1
Accession NZ_CP041426
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag FNE85_RS24260 Protein ID WP_000072677.1
Coordinates 12787..13308 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag FNE85_RS24255 Protein ID WP_001711191.1
Coordinates 12510..12779 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS24230 7925..9274 - 1350 WP_161623757.1 AAA family ATPase -
FNE85_RS24235 9318..10058 - 741 WP_096127582.1 hypothetical protein -
FNE85_RS24240 10343..11110 + 768 WP_000342417.1 hypothetical protein -
FNE85_RS24245 11163..11516 - 354 WP_160378290.1 hypothetical protein -
FNE85_RS24250 11522..12190 - 669 WP_000161228.1 AAA family ATPase -
FNE85_RS24255 12510..12779 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
FNE85_RS24260 12787..13308 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
FNE85_RS24265 13477..13728 - 252 WP_161623758.1 hypothetical protein -
FNE85_RS24270 13730..14422 - 693 WP_161623759.1 hypothetical protein -
FNE85_RS24275 14436..14759 - 324 WP_000064175.1 hypothetical protein -
FNE85_RS24280 15130..16170 - 1041 WP_096127586.1 phage tail protein -
FNE85_RS24285 16561..17007 - 447 Protein_19 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..114158 114158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T128433 WP_000072677.1 NZ_CP041426:12787-13308 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T128433 NZ_CP041426:12787-13308 [Escherichia coli]
GTGAGCAATACGACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGTACTCAAGAAG
AAAGGCCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCACGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT128433 WP_001711191.1 NZ_CP041426:12510-12779 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp

>AT128433 NZ_CP041426:12510-12779 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATAACCCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTAAAGGTTATAGAACAACATCGAAGAC
TGGTTCTGAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGATAAGCTGAAACGA
GCTGCTAATCGTCTGAAAAGCATGGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References