Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4082989..4083539 Replicon chromosome
Accession NZ_CP041425
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag FNE85_RS20145 Protein ID WP_001160971.1
Coordinates 4082989..4083303 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag FNE85_RS20150 Protein ID WP_000125566.1
Coordinates 4083306..4083539 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS20120 4078664..4079950 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
FNE85_RS20125 4079950..4080939 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
FNE85_RS20130 4080936..4081757 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
FNE85_RS20135 4081760..4082137 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
FNE85_RS20140 4082144..4082992 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
FNE85_RS20145 4082989..4083303 - 315 WP_001160971.1 CcdB family protein Toxin
FNE85_RS20150 4083306..4083539 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FNE85_RS20155 4083625..4084104 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
FNE85_RS20160 4084296..4085966 - 1671 Protein_3957 glutathione-regulated potassium-efflux system protein KefC -
FNE85_RS20165 4086082..4086777 - 696 WP_137466630.1 protein mom -
FNE85_RS20170 4086728..4086913 - 186 WP_001557410.1 Com family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T128430 WP_001160971.1 NZ_CP041425:c4083303-4082989 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T128430 NZ_CP041425:c4083303-4082989 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT128430 WP_000125566.1 NZ_CP041425:c4083539-4083306 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT128430 NZ_CP041425:c4083539-4083306 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References