Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2300962..2301600 Replicon chromosome
Accession NZ_CP041425
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag FNE85_RS11250 Protein ID WP_096129258.1
Coordinates 2300962..2301138 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FNE85_RS11255 Protein ID WP_001270286.1
Coordinates 2301184..2301600 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS11230 2296581..2297795 - 1215 WP_161623654.1 BenE family transporter YdcO -
FNE85_RS11235 2297848..2298384 + 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
FNE85_RS11240 2298457..2300418 + 1962 WP_096129257.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FNE85_RS11245 2300510..2300740 - 231 WP_000494244.1 YncJ family protein -
FNE85_RS11250 2300962..2301138 + 177 WP_096129258.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FNE85_RS11255 2301184..2301600 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FNE85_RS11260 2301679..2303085 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FNE85_RS11265 2303330..2304474 + 1145 Protein_2205 ABC transporter substrate-binding protein -
FNE85_RS11270 2304492..2305505 + 1014 WP_161623655.1 ABC transporter ATP-binding protein -
FNE85_RS11275 2305506..2306447 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2295393..2296541 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6696.78 Da        Isoelectric Point: 10.6026

>T128419 WP_096129258.1 NZ_CP041425:2300962-2301138 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLCKAILKQLGLS

Download         Length: 177 bp

>T128419 NZ_CP041425:2300962-2301138 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGTGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT128419 WP_001270286.1 NZ_CP041425:2301184-2301600 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT128419 NZ_CP041425:2301184-2301600 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References