Detailed information of TA system TA128418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1890923..1891394
• Replicon: chromosome
• Accession: NZ_CP041425
• Organism: Escherichia coli strain STEC388

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FNE85_RS09090
• Protein ID: WP_001303511.1
• Coordinates: 1890923..1891201 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FNE85_RS09095
• Protein ID: WP_001302048.1
• Coordinates: 1891203..1891394 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE85_RS09055	1887677..1887880	-	204	WP_064484537.1	DUF1482 family protein	-
FNE85_RS09060	1887877..1888065	-	189	WP_064484538.1	hypothetical protein	-
FNE85_RS09065	1888076..1888930	-	855	WP_096128846.1	Rha family phage regulatory protein	-
FNE85_RS25735	1889501..1889776	+	276	WP_000102678.1	hypothetical protein	-
FNE85_RS09075	1889754..1890074	-	321	WP_000394534.1	hypothetical protein	-
FNE85_RS09080	1890097..1890315	-	219	WP_086590109.1	hypothetical protein	-
FNE85_RS09085	1890475..1890630	-	156	WP_001678562.1	DUF1391 family protein	-
FNE85_RS09090	1890923..1891201	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNE85_RS09095	1891203..1891394	-	192	WP_001302048.1	hypothetical protein	Antitoxin
FNE85_RS09100	1891415..1891786	-	372	WP_001169685.1	hypothetical protein	-
FNE85_RS09105	1891884..1892186	+	303	WP_000172735.1	transcriptional regulator	-
FNE85_RS09110	1892183..1892608	+	426	WP_096128847.1	Rha family transcriptional regulator	-
FNE85_RS09115	1892680..1893699	+	1020	WP_140432539.1	phage replisome organizer	-
FNE85_RS09120	1893611..1894153	+	543	WP_074430107.1	replication protein	-
FNE85_RS09125	1894188..1894949	+	762	WP_140432540.1	DUF1627 domain-containing protein	-
FNE85_RS09130	1894965..1895387	+	423	WP_096128850.1	DUF977 family protein	-
FNE85_RS09135	1895384..1895683	+	300	WP_096128851.1	DUF4406 domain-containing protein	-
FNE85_RS09140	1895730..1896140	+	411	WP_042004587.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2A / stx2B	1881188..1911527	30339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T128418 WP_001303511.1 NZ_CP041425:c1891201-1890923 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T128418 NZ_CP041425:c1891201-1890923 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGCTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT128418 WP_001302048.1 NZ_CP041425:c1891394-1891203 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT128418 NZ_CP041425:c1891394-1891203 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0